For behavioral analyses, adult flies (2–5 days-old) were grown as above, immobilized under light CO2 anesthesia, separated by sex and genotype, and placed into fresh food vials (25 flies/vial for eRING, 11 flies/vial for sedation) overnight at 25°C/60% relative humidity. All comparisons between groups were based on flies that were grown, handled and tested side-by-side. Each vial of flies represents n=1. All behavioral assays were performed at 23–25°C and 50–55% relative humidity under standard laboratory lighting. The experimenter was blind to genotype in all studies.
