Medical School (Boston, MA, U.S.A.) and backcrossed for 7 generations to the w[A] control to normalize their genetic background. An additional w1118 genetic background strain (w[VDRC]) and UAS-RNAi transgenic strains to manipulate white (v30033 and v30034), Clic (v105975), Cnx14D (v5597) and ph-p (v50024) were obtained from the Vienna Drosophila RNAi Center (Vienna, Austria). An X chromosome harboring both the elav-Gal4 driver and the v30034 RNAi transgene (elav-Gal4,v30034) was generated via meiotic recombination. A stock homozygous for a wild-type w allele (w+) in the w[A] background was generated by backcrossing a Canton-S X chromosome to w[A] for 7 generations. Unless otherwise indicated, all strains contained a w1118 X chromosome and all transgenic lines contained mini-w. The previously described scbVol2, AlkMB06458, aru8.128, hppyKG5537, NPFR1-Gal4 and NPFR1-RNAi strains (Bhandari et al., 2009; Corl et al., 2009; Eddison et al., 2011; Lasek et al., 2011; Wen et al., 2005) and control genetic backgrounds were kindly provided by Ron Davis, Ulrike Heberlein and Ping Shen.
