Flies were grown on food medium (10% sucrose, 3.3% cornmeal, 2% yeast, 1% agar) supplemented with active dry yeast, 0.2% Tegosept (Sigma Chemical Co., St. Louis, MO, USA) and antibiotics (0.5 μg ampicillin, 0.1 μg tetracycline, 0.625 μg chloramphenicol per 10 ml of food) at 25°C/60% relative humidity with a 12 hour light/dark cycle. The w1118 control strain isogenic for the X, 2 and 3 chromosomes used in these studies (a.k.a. w[A]) was obtained from the Drosophila Stock Center (stock# 5905, Bloomington, IN, U.S.A.). The elavC155-Gal4 driver (elav-Gal4), Clic (G4072 and EY04209), Akap200 (EP2254, c01373, d01782, d03938, d07255, EY04645 and EY12242), thickveins (7, 8, d07811, f02766, f03305, c06013 and KG05071), wishful thinking (d02492, e00566 and e01243) and baboon (c04263, c05710, k16912) strains were obtained from the Bloomington Drosophila Stock Center (Bloomington, IN, U.S.A.) or the Exelixis Collection at the Harvard Medical School (Boston, MA, U.S.A.) and backcrossed for 7 generations to the w[A] control to normalize their genetic background. An additional w1118 genetic background strain (w[VDRC]) and UAS-RNAi transgenic strains to manipulate white (v30033 and v30034), Clic (v105975), Cnx14D (v5597)
