The data in Fig. 3a illustrate the time course of the ethanol withdrawal response for the High and Low lines at S5. The peak response in the High line occurred 6–8 h after the injection of ethanol (4 g/kg, IP). Naive S5 mice were challenged with 60 mg/kg (IP) of pentobarbital. The time course of the withdrawal response is illustrated in Fig. 3b. The difference in the CAUC between the High and Low lines was 7.0±1.3 vs 1.6±0.56 (p<6 × 10−4); the CMAX was also significantly different (2.5±0.31 vs 0.82±0.31, p< 1 × 10−3). Ethanol naive S5 animals were also tested for ethanol consumption using the standard protocol (see “Materials and methods”). Average consumption in the High and Low lines (10% ethanol, two-bottle choice, 24-h continuous access) was 0.98±0.29 vs 1.2±0.31 g/kg, p>0.5; data not shown).
