The segregation of the High and Low lines for ethanol consumption is illustrated in Fig. 4. The ANOVA revealed a significant effect for line (F3,414=12, p<3 × 10−7), generation (F3,414=13, p<3 × 10−8), and the line × generation interaction (F3,414=11, p<1 × 10−6). The post hoc analysis revealed a significant difference between the High and Low lines at S2 (5.1±0.62 vs 1.5±0.23 g/kg, p<8 × 10−3), at S3 (7.8±0.67 vs 1.7±0.39 g/kg, p<3 × 10−5), and at S4 (9.1±0.71 vs 1.4±0.22 g/kg, p<3 × 10−5). Segregation for preference paralleled the segregation for consumption—the ANOVA revealed a significant line × generation effect interaction (F3,414=14, p<1 × 10−8); at S4, preference (volume of 10% alcohol consumed/total fluid consumed) was significantly different between the High and Low lines (0.47±0.032 vs 0.081±0.027, p<3 × 10−5); although to a lesser degree, preference was also significantly different (p<5 × 10−4 or better) at S2 and S3. The ANOVA for total volume consumed revealed a significant effect for line (F3,414=8.2, p<3 × 10−5) but not generation or the line × generation interaction. On average, the difference in total fluid consumption between the High and Low lines was 6.10±0.15 vs 5.81±0.07 ml, p<0.01).
