Chunk #33 — Materials and Methods — Genotyping

Source

Sorting nexin 27 regulation of G protein-gated inwardly rectifying K⁺ channels attenuates in vivo cocaine response.

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yes

Text

Tail biopsies were collected from animals at weaning (>P21) and PCR genotyping was performed using the following primers: SNX_loxP_geno_F: 5’-AAAGGGCTGGGCGGTAGTGG-3’SNX_loxP_geno2_R: 5’-CAGGGCCCAGATCATTCAACACTTC-3’Flp_geno_F: 5’-ACCATAGGGTTGATGAGATGGCCAA-3’Flp_geno_R: 5’-ACAGCATGACTTGGCTGACATACGTG-3’Cre320 F: 5’-GAACC TGATG GACAT GTTCA GG-3’Cre320 R: 5’-AGTGC GTTCG AACGC TAGAG CCTGT-3’Cre_myo internal control F: 5’-TTACG TCCAT CGTGG ACAGC-3’Cre_myo internal control R: 5’-TGGGC TGGGT GTTAG CCTTA-3’