We hypothesized that that epigenetic disturbances involving DNA methylation are associated with parental THC exposure in the subsequent generation, and that these changes affect gene pathways with relevance to previously characterized behavioral impairments (Szutorisz et al, 2014). To test this, we performed genome-wide DNA methylation analysis using Enhanced Reduced Representation Bisulfite Sequencing (ERRBS) in the nucleus accumbens (NAc)—the ventral subregion of the striatum most linked to addiction vulnerability—of adult F1 offspring. Being the largest study of its kind to date, these data provide novel insight into potential drug-related cross-generational epigenetic effects.
