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Chunk #4 — Materials and Methods — Drugs

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Genome-Wide DNA Methylation Profiling Reveals Epigenetic Changes in the Rat Nucleus Accumbens Associated With Cross-Generational Effects of Adolescent THC Exposure.
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Δ9-THC (50 mg/ml in ethanol solution) was evaporated under nitrogen gas, dissolved in 0.9% NaCl (saline) containing 0.3% Tween 80 to a concentration of 0.75 mg/ml (Dinieri and Hurd, 2012). Control ‘vehicle' (VEH) solution was saline containing 0.3% Tween 80.