Lentivirus encoding TAF15 and LINC00665 was generated with a pLenti6.3/V5eDEST Gateway vector kit (Life Technologies). sh-MTF1 and sh-YY2 were ligated into the LV3-CMV-GFP-Puro vector (GenePharma), respectively. Thus, target lentivirus vectors were generated to obtain the stable expressing cells of precursor (pre-)TAF15, pre-LINC00665, and sh-MTF1 or sh-YY2. Four-week-old BALB/c athymic nude mice were purchased from Beijing Vital River Laboratory Animal Technology (Beijing, China). These nude mice were divided into seven groups: control, TAF15+, LINC00665+, MTF1−, YY2−, TAF15+ + LINC00665+ + MTF1−, and TAF15+ + LINC00665+ + YY2− groups. 3 × 105 (100 μL) cells were injected subcutaneously into the right limb of nude mice. We observed and recorded the tumor formation time and tumor formation of nude mice in each group, calculating the tumor formation rate and detecting the weight and volume of transplanted tumor to draw the tumor growth curve [tumor volume (mm3) = (longest diameter × shortest diameter)2 × 0.5]. The number of survived nude mice was recorded, and survival analysis was performed using a Kaplan-Meier survival curve.
