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Chunk #17 — Materials and Methods — Procedure

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A cis-eQTL in OPRM1 is Associated with Subjective Response to Alcohol and Alcohol Use.
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Participants attended a preliminary laboratory session during which they completed all retrospective self-report measures (i.e., SRE, ASQ, and alcohol consumption items). The alcohol challenge protocols were administered during a second laboratory session approximately one to three weeks later (M = 19.1 days). Participants were instructed to eat a light meal 2 to 4 hours prior to their appointment time. Upon arrival, all individuals provided informed consent and either took a urine stream pregnancy test (women) or were asked to empty their bladder (men). Participants then completed a baseline assessment of subjective effects of alcohol, which included the BAES for stimulation and sedation, as well as a subjective intoxication rating. This assessment was followed by random assignment to receive an active placebo (diluted [10-proof] vodka and tonic water; 0.04 g/kg ethanol [EtOH]) or alcohol beverage (100-proof vodka and tonic water; 0.80 g/kg EtOH for men [0.72 g/kg for women]) with target peak breath alcohol concentration (BrAC) of 0.09%. The contents of the active placebo or alcohol beverage were divided into 3 equivalently sized drinks to be consumed over 8 minutes per drink.