both hMOPR variants (Fig. 6A). With their peak levels as 100%, the levels of the mature forms of A118/N40-hMOPR and G118/D40-hMOPR were quantified at various time points (Fig. 6B) and their half-lives were determined. As evident in Fig. 6B, the fully glycosylated G118/D40-hMOPR was degraded faster than the A118/N40-hMOPR counterparts with half-lives of 11.6±1.9 h (n=3) and 27.7±5.5 h (n=3), respectively (p<0.05) (Table 1). The levels of the precursors of A118/N40-hMOPR and G118/D40-hMOPR were also quantified (Fig. 6B). With their initial levels at 0 h as 100%, the precursors of the two MOPR variants had similar half-lives (1.3±0.2 vs 1.2±0.2 h, n=3) (Table 1).
