To investigate CNV influence on volume variations and behavior, the brain volume component loadings were used in an unbalanced one way ANOVA to find any associated CNV regions. No region passed a Bonferroni corrected significance threshold of p < 4 × 10−5. However, one CNV region located at chromosome 11 q14.2 was found, with p = 0.0043, showing differences in the brain volume and AUD severity components loading coefficients, p < 0.05. This region is approximately 2000 bp (85981702–85984049) long with twelve subjects having homozygous deletions; six have hemizygous insertions and 265 subjects having no variation in this region. The differences in the brain volume loading coefficients are shown in Fig. 4; deletion carriers have significantly, p < 0.05, higher median loading coefficients than insertion carriers or subjects without variation. Similarly, insertion carriers have significantly lower, p < 0.05, median loading coefficients than deletion carriers or subjects with no variation. There is a significant, p < 0.05, negative slope associated with these differences indicating the possible presence of a genetic dosage effect. The high loading coefficients of this brain volume
