Two analysis methods were used to find BRGs for which the spatial expression in the AHBA is related to the progression of the disease: (i) Pearson’s correlation between gene expression and labels 1–6 according to their assignment to one of the Braak stage-related regions R1–R6, and (ii) differential expression between Braak stage-related regions R1 and R6. As the expression values were log2-transformed, the mean difference between two regions was interpreted as the FC. Genes were assigned as BRGs based on the overlap of the top 10% (2001) ranked genes with: (i) highest absolute correlation between gene expression and Braak stage labels, (ii) highest absolute FC between R1 and R6, and (iii) lowest Benjamini-Hochberg (BH) corrected P-value of the FC.
