For assessing neuronal excitability, iN co-cultured with mouse glia on MatrigelTM coated 10 mm glass coverslips were transduced with lentivirus expressing hSyn-GCaMP6f at least 2 weeks prior to imaging to ensure robust expression. All fluorescence imaging experiments were performed in ~50 DIV (days in vitro) iNs. Details of the protocol and analysis are found in the Supplementary Methods.
