FSW-hSyn-GCaMP6f assembly was described previously [33]. FUGW-KCNJ6-mCherry was assembled using lentiviral backbone from FUGW (AddGene #14883), KCNJ6 coding sequence amplified from human neuron cDNA (forward primer: agccaggaaaagcacaaaga, reverse primer: ggggagaagagaagggtttg), and mCherry with a nuclear localization signal from pME-nlsmCherry (a gift from Dr. Kelvin Kwan). During construction, the GIRK2 protein-coding sequence was tagged with a 3xHA tag and linked to mCherry with a T2A “self-cleaving” element.
