Groups of 7 male mice at ~8 weeks of age were used for AAV8 expression studies. Mice received 1 × 1012 viral particles via intraperitoneal injection. Blood was collected prior to injection and at 2 weeks post-injection via retro-orbital bleed, following a 4-hour fast. Mice were sacrificed at 2 weeks post-injection and livers were collected. Gene overexpression was confirmed by qRT-PCR, as described below. Plasma triglyceride, HDL cholesterol, and ALT levels were measured on individual samples using analytical chemistry (Infinity reagents; Thermo Fisher Scientific) on a Cobas Mira Autoanalyzer (Roche Diagnostic Systems). ALT levels were checked to confirm that the lipid phenotypes were not influenced by liver damage (data not shown).
