Chunk #38 — MATERIALS AND METHODS — Live imaging and analysis of Dlxi1/2b::eGFP+ cell migration

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Assembly of functionally integrated human forebrain spheroids.

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For imaging of Dlxi1/2b::eGFP+ cells, E17–E18 slices were placed on inserts and infected with Dlxi1/2b::eGFP lentivirus after 24 hrs. The slices were imaged 2 days later using a Leica SP8 confocal microscope (see above).