For measuring the branch to soma length ratio of human cells on mouse slices, hSSs infected with Dlxi1/2b::eGFP lentivirus were dissociated and placed on top of E13–14 mouse slices, which were placed on cell culture inserts 8–24 hrs before. The hSS-derived Dlxi1/2b::eGFP+ cells were imaged with the Leica SP8 confocal microscope system at least 48 hrs later.
