Postmortem human brain tissue from CN, PUT and VS of a total of 48 individuals with AUD and 51 control individuals (68% male) was obtained from the New South Wales Tissue Resource Centre at the University of Sydney. The Ethics Committee II of the University of Heidelberg approved the study (reference number 2021–681). After quality control (QC), the total sample sizes for each brain region were NCN = 71, NPUT = 77 and NVS = 63. Phenotypic information was assessed by next-of-kin interviews. Inclusion criteria for this study were: age > 18 years, Western European Ancestry, no history of severe psychiatric or neurodevelopmental disorders, or SUDs other than AUD and nicotine use disorder or smoking. AUD was defined as meeting DSM-IV criteria for alcohol dependence and consuming 80 g of alcohol a day or more (control group: <20 g/day). Descriptive information can be found in Table 1 and Supplementary Table S1.Table 1Descriptive statistics of demographic data.CharacteristicCasesControlspN4851Age, years55.58 (10.62)57 (10.64)0.51Sex (M/F)31/1737/14pH-value6.53 (0.26)6.65 (0.25)0.026*PMI (hours)37.07 (15.79)30.7 (15.57)0.047*Blood alcohol level (N)70Blood alcohol level (g/100 ml)0.21 (0.21)Smoking (yes/%)32 (66.7%)12 (23.5%)<0.001*Samples per brain region Caudate
