As an initial characterization of the role for Clic4 in ethanol responses in mammals, we used genomic studies across the BXD genetic panel of mice to determine whether basal expression of Clic4 in PFC correlated with ethanol-related behaviors in the GeneNetwork web resource (www.genenetwork.org). Through this analysis we found that Clic4 PFC basal expression correlated positively with low dose (2 g/kg) ethanol-induced locomotor activation (Figure 1B) and inversely with high dose (4 g/kg) ethanol-induced ataxia (Figure 1C) in mice. Our analysis of data from human and rodent studies thus suggests that Clic4 or related genes might be important determinants of acute level of response to ethanol across a range of species.
