strains differ widely in terms of multiple behavioral responses to acute or chronic ethanol, as well as ethanol consumption (Metten et al., 1998). We also found that Clic4 was in other published datasets for genes associated with ethanol drinking preference in mice (Mulligan et al., 2006) and genes with altered expression in frontal cortex of alcoholics (Mayfield et al., 2002). Furthermore, Clic4 is located within a confirmed quantitative trait locus (QTL) for ethanol drinking behavior on distal mouse chromosome 4 (Tarantino et al., 1998) and we also found that ethanol treatment (4 g/kg × 4 h) increased expression of Clic4 in PFC of DBA/2J mice (Figure 1A; *, two-sample t-test; t(6)=3.091, p=0.027, n=4 for EtOH and 3 for Saline), validating our prior microarray data (Kerns et al., 2005).
