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Chunk #31 — Methods — Estimation of lentivirus titer

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Habenular α5 nicotinic receptor subunit signalling controls nicotine intake.
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Viral supernatant titres were determined using the Lentivector Rapid Titer Kit from System Biosciences, according to the manufacturer's instructions. The number of infectious units per ml of supernatant (IFU ml−1) was calculated as follows: Multiplicity of infection (MOI) of the sample × the number of cells in the well upon infection × 1,000 / μl of viral supernatant used.