We used the solution-based hybridization targeted capture technology (SureSelectXT, Agilent) to target entire genes and ±5kb of their flanking regions. In this method, a library of synthetic oligonucleotides (baits) complementary to the sequence of interest is custom designed and manufactured (Gnirke et al., 2009). These baits are then used to pull down the desired genomic regions from fragmented genomic DNA samples. Library design and bait tiling were carried out using Agilent eArray.
