Libraries described as 'low-input Fisher et al. modified with Kapa Biosystems reagents' were made as described above except library construction and PCR reagents were obtained from Kapa Biosystems. DNA fragment end repair, A-base addition, and adapter ligation reactions were performed according to the manufacturer's recommendations (Kapa Biosystems, MA, catalog number KK8201) but utilizing the 'with-bead' SPRI-based clean up method in Fisher et al. Library enrichment with Kapa HiFi enzyme (catalog number KK2102) was performed as follows: the entire unenriched product was enriched in a reaction volume of 60 µl in the presence of 1× Kapa HiFi HF buffer, 0.4 mM each dNTP, 0.8 µM of each enrichment primer, and 1 unit of Kapa HiFi enzyme. Kapa HiFi PCR enrichment was performed for 8 cycles with the following cycling parameters: 98°C for 45 seconds; 8 cycles of 98°C for 15 seconds, 60°C for 30 seconds, 72°C for 30 seconds; 72°C for 1 minute.
