cells were collected by FACS and plated at about 5,000 cells per well in a 6-well plate on irradiated mouse embryonic fibroblasts (MEFs). By 7-10 days, colonies were visible and hand-picked for screening. Three iPS cell clones were selected: C12, which had no evidence of editing to be used as a negative control; D11 and A10, which both had homozygous edits to produce rs1799971 major allele (AA). In all gene-targeted cell lines, sequencing confirmed these edits and that all predicted off-target sites were unchanged.
