To date very few experimental intervention studies have been performed with human subjects. The first, Wurm et al. (2007) (78) examined circulating CTRP3 levels before and 2 hours after a glucose load (n = 20, 14 males and 6 females) and observed no change. However, this study occurred before there were reliable ELISA’s developed for CTRP3 and examined CTRP3 levels solely though immunoblot analysis. Further complicating the results from this study is that although they reported the supplier (R&D Systems) they did not include the catalog number for the antibody used and they reported band migration of 50 kDa for CTRP3 on a denaturing SDS-PAGE. CTRP3 has a predicted and gel migration ~30 kDa and although the posttranslational modifications for CTRP3 could result in a higher than predicted migration pattern on a SDS gel, this has not been observed by other researchers (1, 43, 51, 70, 75). Indeed, the antibody for human CTRP3 from R&D Systems (R and D Systems Cat#AF7925, RRID:AB_2619735) also reports detecting a band at ~30 kDa. Moreover, using an ELISA based method Ban et al. (2014)
