other researchers (1, 43, 51, 70, 75). Indeed, the antibody for human CTRP3 from R&D Systems (R and D Systems Cat#AF7925, RRID:AB_2619735) also reports detecting a band at ~30 kDa. Moreover, using an ELISA based method Ban et al. (2014) observed that in type 2 diabetic patients CTRP3 levels decreased from ~150 to 50 ng/ml in response to a 2-hour oral glucose load (5). In light of these data and the progress within the past 9 years in CTRP3 antibody and ELISA development, the effects of glucose on CTRP3 levels in healthy human subject population should be reexamined. In addition, due to the potential role of CTRP3 in lipid metabolism, experiments should also examine the effects of acute lipid loading on circulating CTRP3 levels.
