Oligodendrocytes wrap myelin sheets around axons to support long-range neurotransmission and were previously analyzed by scRNA-seq (Marques et al., 2016). The greatly increased sampling depth in the present study did not reveal any additional, clearly distinct subtypes of mature oligodendrocytes beyond those we had already described. Furthermore, OPCs remained a single cluster, with only a distinction between cycling and non-cycling OPCs (Figures 4A and 4B). Immature oligodendrocyte lineage types (OPC, committed oligodendrocyte precursor [COP], and newly formed oligodendrocyte [NFOL]) were intermingled in all tissues (except for a technical batch effect in the medulla and pons; Figure 4B). Note that although the oligodendrocyte lineage clusters aligned well with those previously published (Figure S1H; Marques et al., 2016), there was not a perfect correspondence, and subtype labels in this paper do not correspond exactly to those previously described.
