We validated the identity and distribution of astrocyte cell types using RNA FISH (RNAscope), which was consistent with the Allen Mouse Brain Atlas (Figures 3F and S2). Co-staining of Mfge8 and Agt on a sagittal section revealed a distinct border separating the telencephalon from the diencephalon. The olfactory bulb and cerebellum were enriched with their local astrocytes ACOB and ACBG marked by Islr and Gdf10, respectively. Moreover, we validated the distribution of neurotransmitter transporters with Slc6a11 (also known as GAT3, the GABA reuptake transporter), highly expressed in the olfactory and the non-telencephalon astrocytes (but not in cerebellum), and Slc6a9 (glycine transporter GLYT1), with similar pattern but lower olfactory expression and a higher expression in the cerebellum.
