Genomic DNA was prepared using Zymo gDNA columns (Zymo Research), and specific regions were amplified by PCR using primers listed in Table S1 using Q5 mastermix (New England Biolabs). PCR fragments were cloned using the StrataClone Blunt PCR cloning kit (Agilent). Sequencing was performed by GenScript. ATM mutations were compared with annotations in the LOVD database (http://atm.lovd.nl). TCRβ junction sequences were identified by amplification with the Jβ1 family of primers and the Vβ pan primer from Assaf et al. (2000). Amplified fragments were cloned into the Stratagene Blunt PCR cloning kit (Agilent) and sequenced with an M13R vector-specific primer.
