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Chunk #54 — Online Methods — Gene Expression and Differential Analysis

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The neuron-specific chromatin regulatory subunit BAF53b is necessary for synaptic plasticity and memory.
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Differential transcriptional analyses were performed using CyberT32,33 across each pair of groups: WT HC, WT Beh, Baf53b+/− HC, and Baf53b+/− Beh. Additional control analyses were performed using CASAVA. For different threshold values the corresponding number of genes predicted to be differentially regulated, up-regulated, or down-regulated was examined. Enrichment for specific tissue expression, GO terms35, and KEGG pathways36,37 was assessed using DAVID38.