Quantitative RT-PCR (qRT-PCR) was performed using methods previously described 28,51. Tissue was collected from dissections of dorsal hippocampal hippocampus and stored in RNALater (Invitrogen, AM7020) at −80C until processed. RNA was isolated using RNeasy minikit (Qiagen, 74104). cDNA was made from 500-1000ng total RNA using the Transcriptor First Strand cDNA Synthesis kit (Roche Applied Sciences, 04379012001). Primers and probes were derived from the Roche Universal ProbeLibrary (Supplemental Table S13) and used for multiplexing in the Roche Light- Cycle 480 II machine (Roche Applied Sciences). All values were normalized to gapdh expression levels. Analysis and statistics were performed using the Roche proprietary algorithms and REST 2009 software based on the Pfaffl method58,59.
