Total RNAs from amygdala (AMY), nucleus accumbens (combined core and shell, NAC) and prefrontal cortex (PFC) were shipped to the Molecular Genomics Core Facility at Moffitt Cancer Center (Tampa, FL). Samples were biotin-labeled using the FlashTag Biotin HSR RNA Labeling Kit (Affymetrix, Santa Clara, CA) and hybridized to GeneChip miRNA 3.0 arrays (Affymetrix, Santa Clara, CA) according to manufacturer instructions. This platform used annotations from miRbase version 17 and contained probe sets for over 19,000 mature microRNAs from 153 species. Transcript abundance was measured by fluorescent intensity after scanning with the Affymetrix GCS3000 scanner and generation of cel files with Affymetrix AGCC v3 software. MicroRNA array data have been submitted to the NCBI Gene Expression Omnibus (GEO) (http://www.ncbi.nlm.nih.gov/geo/) under accession number GSE90608. Arrays were hybridized with material from a single animal (no pooling); thus, 144 samples (16 animals x 3 tissues x 3 time points) were profiled.
