Finally, we examined the inter-hemispheric cortico-cortical FCs for each layer (i.e., between the left and right brain regions of the same area) and compared the FC results before deconvolution (Fig. 4c) with FC results after deconvolution (Fig. 8c). Before deconvolution, only the inter-hemispheric cortico-cortical FCs for layer III were significantly greater than layer VI (Fig. 4c). However, after deconvolution, FCs between homologous regions in layers I–III were significantly greater than in layers IV–VI (Fig. 8c). Generally speaking, the inter-hemispheric mean correlations in superficial layers were higher compared to those deeper layers [71].