DS-epi1 staining was performed using immunopurified anti-DS-epi1. Briefly, TE-1 cells were grown in chamber slides, fixed with methanol for 10 min, permeabilized for 10 min in 0.2% Triton X-100, and blocked for 1 h with 5 % BSA in TBS. Immunopurified anti-DS-epi1 (2 μg/ml) and anti-GM130 (1:100) antibodies were incubated overnight at 4 °C and visualized using goat anti-rabbit IgG AF488 (Invitrogen A1100) and goat anti-mouse IgG AF546 (Invitrogen A11003), respectively, at 1:200 dilutions. The presence of IdoA on the cell surface was visualized using a single chain phage display antibody (GD3A12) that specifically recognizes DS (18). Cells were fixed with methanol for 2 min and stained as previously described by Li et al., 2008 (9). Immunostaining of pFAK and F-actin was performed after a wound scratch assay in chamber slides. Cells were fixed in 4% PFA, permeabilized in Triton 0.1%, blocked in 1% BSA for 30 min and stained with anti-pY397FAK (1:1000) overnight at 4 °C in 1% BSA. pFAK was visualized with goat-anti mouse-AF488 at 1:500 dilution (Invitrogen) and subsequently counterstained with phalloidin-TRITC (P-1951, Sigma). HGF surface staining
