Chunk #14 — Results — Human astrocyte co-cultures show expression of both GABAergic and glutamatergic vesicular proteins

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Sustained synchronized neuronal network activity in a human astrocyte co-culture system.

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To verify whether the functional activity is also reflected by morphological correlates of connectivity we performed an automated analysis of synaptic marker expression in hiPSC-derived cortical neurons (Fig. 6a,b). Expression of glutamatergic and GABAergic vesicular proteins vGLUT1 and GAD65 complemented the contribution of both neurotransmitter pathways to the observed neuronal network function (Fig. 6c). At all three time points, similar levels of vGLUT1 and similar levels of GAD65 expression per neurite surface were observed.