LDSR was used to estimate the genetic correlation between cannabis use disorder and a broad measure of maximum cannabis use frequency. Linear regression was then used to examine the extent to which polygenic risk scores (PRS) for cannabis use disorder predicted a pseudocontinuous measure of self-reported cannabis use frequency, while covarying for age, sex, and 20 ancestral principal components (appendix p 16). PRSice-238 was also used to do gene-set enrichment using gene sets and pathways from the Molecular Signatures Database.39
