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Chunk #68 — Methods — Differential gene and cellular state expression analysis in humans

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Single nuclei transcriptomics in human and non-human primate striatum in opioid use disorder.
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We calculated the differential expression in chronic morphine exposure vs. non-morphine treat rhesus macaque subjects. We used one linear model with a nested variable capturing the interaction between cell type, morphine treatment and matching stats.\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${{{{{\rm{Gene}}}}}}\_{{{{{\rm{expression}}}}}} \sim 0+{{{{{\rm{Celltype}}}}}}\_{{{{{\rm{Tx}}}}}}+{{{{{\rm{Pair}}}}}}+{{{{{\rm{SVs}}}}}}.$$\end{document}Gene_expression~0+Celltype_Tx+Pair+SVs.