Chunk #20 — Materials and methods — Microscopic analysis and quantification

Source

Permanent impairment of birth and survival of cortical and hippocampal proliferating cells following excessive drinking during alcohol dependence.

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Cells in the mPFC (i.e., Ki-67-, AC-3-, and BrdU-immunoreactive [IR] cells in the cingulate, infralimbic, and prelimbic cortices) and SGZ (Ki-67-, doublecortin [DCX]-, AC-3-, and BrdU-IR cells touching and within three cell widths inside and outside the hippocampal granule cell-hilus border) were quantified with a Zeiss Axiophot photomicroscope (400×) using the optical fractionator method. Cells from each bregma region were summed and multiplied by 9 to give the total number of cells.