Regional fat depots were assessed from CT scans obtained in Pittsburgh on a General Electric 9800 Advantage (General Electric, Milwaukee, WI) and in Memphis on a Siemens Somatron Plus 4 (Siemens, Erlangen, Germany) or Picker PQ2000S (Marconi Medical Systems, Cleveland, OH). A single axial scan (140 kVp, 300 to 360 mAs, 10-mm thickness) was taken at the disk space between the fourth and fifth lumbar vertebrae. Images were transferred to the Reading Center at the University of Colorado Health Sciences Center on optical disc or magnetic tape. Analyses were performed on a SPARC station II (Sun Microsystems, Mountain View, CA) using IDL development software (RSI Systems, Boulder, CO). An outline was traced surrounding the abdominal cavity. The adipose tissue density range was determined with a bimodal image distribution histogram for each participant. Visceral fat was defined as the area of all adipose tissue within the abdominal cavity with exclusion of the muscle region, calculated by multiplying the number of pixels within this range by a single pixel area. Abdominal subcutaneous fat was defined as the difference in the area between