ng/ml). Collected CD43+ iHPCs were then plated for iMGL differentiation as mentioned above. iMGLs were suspended in FACS buffer and incubated with human Fc block (BD Bioscience) for 15 min at 4°C. For detection of microglial surface markers, cells were stained with anti CD11b-FITC clone ICRF44, anti CD45-APC/Cy7 clone HI30 (Tonbo Biosciences), anti CX3CR1-APC clone 2A9-1, anti CD115-PE clone 9-4D, and anti CD117-PerCP-Cy5.5 clone 104D2, ZombieViolet™ live/dead stain, all from BioLegend. Cells were sorted on a FACS Aria II, FACS Aria Fusion (BD Biosciences) and data analyzed with FlowJo software (FlowJo).
