1×105 cells were suspended in FACS buffer (100 μl) and added to Shandon glass slides (Biomedical Polymers) and assembled in a cytology funnel apparatus. Assembled slides containing cells were loaded in a cytospin instrument and centrifuged (500 rpm, 5 min). Slides were allowed to air-dry for two minutes and immediately stained in May-Grunwald stain (100%; Sigma) for 5 min. Next, slides were washed in PBS for 1.5 min and immediately placed in Giemsa stain (4%; Sigma) for 20 min at room temperature. Slides were washed in double-distilled water 6 times and allowed to air-dry for 10 min. Slides were preserved using glass coverslips and permount (Sigma).
