We then obtained association results for SNPs in 194 genomic regions in six independent samples for a total sample size of over 21,000 cases and 38,000 controls(Table 1). The genomic regions for which replication genotypes were sought were identified using LD clumping defined by LD (r2> 0.5) and a minimum P < 1×10−5 in the Sweden-PGC meta-analysis. Only one MHC SNP was included. The Sweden-PGC meta-analysis and replication results were highly concordant with 76.3% of the logistic regression beta coefficients having the same direction of effect (sign test P=1.5×10−17). Indeed, of the top 100 SNPs in the Sweden-PGC meta-analysis, 90% had the same sign in the replication results. This result strongly suggests that many more loci will achieve genome-wide significance with further increases in sample size.
