the cortex, nucleus accumbens, and cerebellum (Fig. 3A–F). No gross differences were observed in the levels and patterns of GFP fluorescence between groups of animals that had been injected with shScr or shLmo3.8 lentivirus. However, the levels of GFP fluorescence varied among individual animals within each group. To quantify the amount of GFP and Lmo3 transcript in individual animals, we performed qPCR using RNA isolated from the forebrain of each founder animal (Fig. 3G). Within the shScr group, GFP expression (normalized to Gapdh expression as a control for input RNA) ranged from 0.08 to 1.92; comparable levels of GFP expression were observed in the shLmo3.8 group (range: 0.03 to 1.64). Infection with shScr did not affect expression of Lmo3, as Lmo3 expression varied from 0.74 to 0.85 in these animals, similar to the levels of Lmo3 observed in animals that did not contain integrated virus (data not shown). In contrast, expression of Lmo3 ranged from 0.41 to 0.86 in shLmo3.8-infected animals, indicating that shLmo3.8 was, at least in some animals, effective in reducing levels of Lmo3 in vivo. Importantly, GFP expression was inversely correlated with Lmo3 expression in shLmo3.8- infected mice (Fig. 3G, R = −0.628, p = 0.029), indicating
