Chunk #29 — METHODS — Immunostaining

Source

A comparison of genetically matched cell lines reveals the equivalence of human iPSCs and ESCs.

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Immunostaining was performed using the following antibodies: α-hTRA-1-81 (330704, BioLegend), Streptavidin APC (17-4317-82, eBioscience) α-hCD90 (328118, BioLegend), α-Sendai viral protein (PD029, MBL International), and α- OCT4 (ASK-3006, Applied StemCell), α-PAX6 (Cat. no. PAX6, DSHB), and α- SOX1 (Cat. no. 4194, Cell Signaling).