Chunk #30 — METHODS — Lactate production assay

Source: A comparison of genetically matched cell lines reveals the equivalence of human iPSCs and ESCs.
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Lactate production assay was done according to Zhong et at41. Lactate concentration was determined with the Lactate Assay Kit (BioVision). O.D. was measured at 570nm, 30 min. after addition of substrate.