Altered loss of righting reflex (LORR) in mice with AAV2 viral vector-mediated expression of Clic4 in brainAAV2 vectors expressing a Clic4-FLAG fusion protein (AAV-CLIC4; panel A) or empty vector (AAV-IRES; panel B) were stereotactically injected into male DBA/2J mouse PFC. Panels A and B show immunohistochemistry results for FLAG epitope primary antibody staining. Immunohistochemistry was done 2 weeks after the last behavioral studies (~9 weeks after viral injections). Panel C shows that Clic4 over-expressing animals (black) had a shorter duration of LORR following 3.8 g/kg IP of ethanol compared to control (grey). Behavioral testing for LORR was done ~7 weeks after viral injections.
