Chunk #5 — Methods — RNA Sequencing and 5′ Rapid Amplification of Complementary DNA Ends

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Expression of ZNF804A in human brain and alterations in schizophrenia, bipolar disorder, and major depressive disorder: a novel transcript fetally regulated by the psychosis risk variant rs1344706.

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After confirming the presence of a truncated transcript of ZNF804A in the commercial fetal and adult brain poly(A)+ mRNA samples, we performed end-to-end PCR in the DLPFC series (Table) to establish the presence of the truncated transcript in an independent sample and in a specific brain region.