Digested glioma cells, about 20,000 cells per well, were seeded into the polycarbonate membrane chambers. When the cell migration ability was measured, the upper chamber contained DMEM high-glucose medium without FBS, whereas the lower chamber contained high-glucose medium with 10% FBS. When the cell-invasive ability was detected, Matrigel was additionally added to the polycarbonate membrane chambers. After 24 h of culture, the cells were removed and cell migration could be observed under a microscope.
