Figure 4 also shows the effects of anandamide alone and in combination with URB597 (10 – 50 nM) on [35S]GTPγS binding (Fig. 4, bottom panel). Emax values for anandamide-induced stimulation were similar with and without addition of URB597 (229.00 ± 6.66 % and 212.90 ± 3.49 %, respectively). In contrast, adding URB597 shifted the anandamide concentration curve to the left, resulting in EC50 values of 2.76 μM for anandamide alone and 0.69 μM for anandamide plus URB597.
